Isolation and characterization of lividomycin A inactivated by Pseudomonas aeruginosa and Escherichia coli carrying R factor.

served in IR spectrum. The inactivated lividomycin A consumed 6.2 moles of periodate for 24 hours as does lividomycin A (5.9 moles). Methanolysis of the inactivated lividomycin A (refluxed in 0.4 N hydrogen chloride in methanol for 6 hours) gave 3'deoxyparomamine, methyl lividotriosaminide phosphate and a small amount of methyl lividobiosaminide phosphate. These results indicate that the inactivated lividomycin A is a monophosphate at either the 2or 5-hydroxyl group of the ribose moiety in lividomycin A. Moreover, results of pmr decoupling experiments indicate that C-2 position in the mannose moiety (2""-H: £4.43, J=1.5, 3.0Hz)and the C-3